Objective Imaging公司的自動化高速掃描玻片影像系統(Surveyor Automated Scanning and Imaging with Turboscan),利用相機硬體觸發同步訊號方式,同時控制自動移動平台的移動速度,可在載台高速移動的狀態下進行連續影像擷取,實現顯微光學中高倍率、高解析、大範圍的影像拼圖掃瞄整合應用。
快速的拍照掃描整個玻片或樣品,讓樣品內的觀察目標,快速的呈現出完整樣貌,讓專業研究者可以在很短的時間內,了解整個樣品細微結構與組織形態,解脫顯微鏡高倍圍觀察的視野侷限,適用病理組織切片、金相組織大範圍掃瞄和半導體晶圓大範圍掃瞄拼圖等應用需求。
TurboScan只需簡單的滑鼠點擊設定,便可以檢測任何想要觀察或確認的玻片位置,對於研究者後續重覆檢測確認、研討、發佈等工作,都可以節省很多的時間。
利基於OASIS Automation Controller與特殊的相機觸發功能,TurboScan提供一套理想的自動化掃片解決方案,包含樣品的定位、掃描、隨機拍照、自動化拍照存檔等等。
結合Prior PL200玻片自動載入機,可容納兩百片玻片,更可以達成大量的自動化玻片掃描工作,適用於大量病理切片顯微影像掃描應用,對於有大量玻片顯微拍照的工作者,是最佳的選擇。
相關應用:玻片掃描、病理切片掃描
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功能特點
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Mosaic Acquisition
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Turboscan for fast continuous scan and acquire
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Standard scan for step and acquire for low light applications
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Snake and raster scanning
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User-defined settle time at each field
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Pause and continue scanning mode
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Grab individual fields into mosaic with key-press
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Acquire at full or reduced resolution
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JPEG compression option
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Image streaming for mosaic sizes limited only by free disk space
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Specimen Map
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Stage and pattern views
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Point and click relocation to position or pattern field
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Real-time graphic display of current stage position on specimen map
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Zoom and pan
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Drag-drop pattern definition
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Acquire and display multiple mosaics at different magnifications
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Scan Patterns
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Calibration
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X and Y calibration factors
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Stored calibration table for multiple lens and secondary optic settings
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Guided camera to stage alignment procedure
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Automatic correlation-based calibration procedure
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Automatic stage orthogonality correction
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Camera Support
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Image setup based on camera capabilities
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Exposure, gain, offset, and gamma control as available
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Automatic and manual white balance
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Color or monochrome acquisition
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Frame-rate shading correction
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Shading correction on live display
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Calibrated graticule overlay on live image
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Grab, copy, save live image
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Microscope Automation
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OASIS-4i XY stage and Z focus
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Software joystick for stage, focus, turret, and lamp control
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Leica Microsystems DM range focus, turret, condenser, and lamp control via RS-232
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Olympus BX-61 focus, turret, condenser, and lamp control via RS-232
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Stage and focus speed defined per objective
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Computer controlled parfocality and illumination defined per objective
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Export / Import
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Workspace save and load of full mosaic, scan pattern and Surveyor envirnoment
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Export mosaic as full resolution bitmap
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Export mosaic as reduced resolution bitmaps
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Export user-selected region of interest
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Print full mosaic or selected region
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Print preview
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Automatic Focus
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Multi-point predictive focus for continuous focus tracking
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Predictive focus setup wizard
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Video autofocus using OASIS-AF module
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Digital camera autofocus using OASIS-DC1 module
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Autofocus parameters stored separately for each objective lens
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展示影片
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範例照片
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Mouse, Transverse Section
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 17 x 20, 340 total
Size at full resolution: 340 x 4.2 MB = 1.4 GB
Scan area: 10.6 x 9.2 mm = 98 sq. mm
Scan time (snake scan with predictive focus and shading correction): 42 secs
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掃瞄後影像全覽
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影像放大細節
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Zebra Fish
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 16 x 17, 272 total
Size at full resolution: 272 x 4.2 MB = 1.2 GB
Scan area: 9.2 x 7.3 mm = 67 sq. mm
Scan time (snake scan with predictive focus and shading correction): 37 secs
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掃瞄後影像全覽
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影像放大細節
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Appendix, Human
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 16 x 18, 288 total
Size at full resolution: 288 x 4.2 MB = 1.25 GB
Scan area: 10.1 x 8.2 mm = 83 sq. mm
Scan time (raster scan with predictive focus and shading correction): 54 secs
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掃瞄後影像全覽
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影像放大細節
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Mouse Foetus, Lateral Section
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 20 x 16, 320 total
Size at full resolution: 320 x 4.2 MB = 1.3 GB
Scan area: 12.5 x 7.4 mm = 93 sq. mm
Scan time (snake scan with predictive focus and shading correction): 46 secs
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掃瞄後影像全覽
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影像放大細節
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Eye Section, Rabbit
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 19 x 23, 437 total
Size at full resolution: 437 x 4.2 MB = 1.8 GB
Scan area: 11.9 x 10.6 mm = 126 sq. mm
Scan time (raster scan with predictive focus and shading correction): 1 min 19 secs
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掃瞄後影像全覽
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影像放大細節
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Human Skin, Showing Follicles
10X Objective / 1X C-mount
Calibration: 0.46 µm/pixel
Scan fields: 15 x 15, 225 total
Size at full resolution: 225 x 4.2 MB = 0.95 GB
Scan area: 9.4 x 6.9 mm = 65 sq. mm
Scan time (raster scan with predictive focus and shading correction): 38 secs
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掃瞄後影像全覽
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影像放大細節
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